FAQ: What is the difference between EnGen® Spy Cas9 NLS (NEB #M0646) and Cas9 NLS, S.pyogenes (NEB #M0641)?

EnGen Spy Cas9 NLS (NEB #M0646) contains two nuclear localization signals located on the N- and C- termini of the protein. In contrast Cas9 NLS, S. pyogenes (NEB #M0641), contains one NLS signal located on the C- terminus of the protein.