FAQ: Is the genome of SHuffle strains available?

Yes. You can read the paper that describes the complete annotated genome of both the K-12 version of SHuffle (C3026) and the B version (C3029) in Genome Announcements (2016) Mar 31;4(2). The annotated complete genome sequences are deposited at EMBL, EBI, under the following assembly accession numbers: NEB T7 express (C2566), CP014268; SHuffle-B (C3029), CP014269; DHB4, CP014270, and its cognate F′ episome, CP014271; and SHuffle K-12 (C3026), CP014272, and its cognate F′ episome, CP014273.