FAQ: What is the reaction temperature range for Rapid PNGase F (non-reducing format)?

Using the Rapid PNGase F Antibody Standard (NEB #P6043), which is a murine anti-MBP monoclonal antibody, isotype IgG2a, effective deglycosylation was observed in 10 minutes at temperature ranging from 48 to 58°C. The optimal temperature for the use of Rapid PNGase F (non-reducing format) is 50°C.