FAQ: What is the difference between Rapid PNGase F and Rapid PNGase F (non-reducing format)?

Rapid PNGase F (non-reducing format) reaction conditions preserve the integrity of disulfide bonds, making it the reagent of choice for mass spectrometry studies of intact deglycosylated multimeric proteins (i.e. antibodies). Whereas, Rapid PNGase F (NEB #P0710) is ideal for downstream N-glycan analysis and profiling applications. In addition, Rapid PNGase F (non-reducing format) follows a 2-step protocol, as it has a strict requirement for a pre-incubation at 75°C.