HomeFAQsHow much of the control RNAs should I use if I want to spike them into my sample?
FAQ: How much of the control RNAs should I use if I want to spike them into my sample?
If you intend to spike the control RNAs into your sample it is essential to use a much smaller quantity than when doing a control IP on them alone. This is because the m6A control RNA is highly modified and the antibody has a high affinity for it. This will result in the m6A control RNA becoming predominant in the sample after IP. Use of 1 µl of a 1:1000 dilution of each control RNA (0.1 fmol of each RNA) is recommended. The control RNAs can be spiked in either before or after fragmentation of the RNA sample. If spiked in after, a concentrated stock of the control RNAs should be fragmented and then diluted prior to addition to the sample.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
