FAQ: How much antibody sample can be deglycosylated with Rapid PNGase F (non-reducing format)?

1 µL of Rapid PNGase F (non-reducing format) in a 10 μL total reaction volume is optimal for rapid deglycosylation of up to 10 μg of antibodies containing both Fc and Fab glycosylation (such as Cetuximab), 15 μg of a mouse monoclonal antibody isotype IgG2a, and 30 μg of less complex antibodies, such as Rituximab. Reactions may be scaled-up linearly to accommodate larger amounts of antibody and/or glycoprotein.