FAQ: How can I test if the Rapid PNGase F (non-reducing format) reaction is complete in 10 minutes?

For a monoclonal antibody, a SDS-PAGE shift can be observed after removal of N-glycans. Typically, this is only a modest change, so it is crucial to run a control along with the actual sample to compare the position on a gel, and determine whether deglycosylation is partial or complete (refer to the “SDS-PAGE” protocol). To detect whether or not minor amounts of glycosylated antibody remain, a more sensitive assay is required. For instance, a proteomic analysis by LS-ESI-TOF will show the different glycosylated and non-glycosylated forms present.