HomeFAQsCan a protease inhibitor cocktail be used in a Rapid PNGase F (non-reducing format) reaction?
FAQ: Can a protease inhibitor cocktail be used in a Rapid PNGase F (non-reducing format) reaction?
Yes. Protease cocktails are compatible with the Rapid PNGase F (non-reducing format) reaction. The following protease inhibitors have been tested: Aprotinin (10 μg/ml final concentration), Benzamidine (1mM final concentration), Pepstatin (10 μg/ml final concentration), Leupeptin (1μM final concentration), EGTA (1 mM final concentration), EDTA (1 mM final concentration) and PMSF (1 mM final concentration). Please note that we do not recommend PMSF as it has the ability to modify basic residues on glycoprotein substrates.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.