FAQ: Can modified nucleotides be used with the HiScribe T7 ARCA mRNA kits?

The HiScribe T7 ARCA mRNA kits are capable of incorporation of 5mCTP and Pseudo-UTP into mRNA. Up to 2.5mM total modified nucleotides can be added into the reaction without impacting the mRNA yield significantly. Other modified UTP and CTP may be used but the yield will vary depending on the property of the nucleotides. Modified GTP and ATP should not be used because they will interfere with capping and tailing efficiency.