FAQ: Can I use T3 RNA Polymerase to make high specific activity radiolabeled probes?

Yes, 3RNA Polymerase, SP6 RNA Polymerase and T7 RNA Polymerase and the HiScribe T7 Kit (NEB #E2040) can be used to make RNA probes. Higher detection sensitivity of nucleic acid can be obtained using an RNA probe due to the greater stability of RNA/DNA hybrids than DNA/DNA hybrids.