HomeFAQsWhat buffers or additives should be avoided when using Rapid PNGase F?
FAQ: What buffers or additives should be avoided when using Rapid PNGase F?
The target protein should be in a solution compatible with Rapid PNGase F activity. Avoid buffers containing SDS, as it inhibits PNGase F. Common stabilizing reagents such as Tween, Triton X-100, NP-40, octyl glucoside and non-detergent sulfobetaines, as well as traces of organic solvents, can prevent optimal rapid deglycosylation.
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