HomeFAQsHow does One-Step RT-PCR kit protocol work for NEB #E5315?
FAQ: How does One-Step RT-PCR kit protocol work for NEB #E5315?
The One-Step RT-PCR reactions allow both cDNA synthesis and PCR amplification
in the same reaction buffer. A thermocycler is programmed so that
cDNA synthesis takes place first (48°C for 15 minutes), then reverse transcriptase
is inactivated while Hot Start Taq Polymerase is activated (94°C for
1 minute), followed by 40 cycles of PCR amplification.