FAQ: Do I need to optimize the reaction components?

The OneTaq One-Step RT-PCR Kit standard reaction conditions (1.6 mM MgCl2 and 250 µM dNTP at 1X) work well for most targets. You may be able to add more MgCl2 or dNTPs to make your reactions work better, but in most cases re-designing your primers may be more effective.