FAQ: Does Bst DNA polymerase have reverse transcriptase activity?

Yes, but it is typically too low for use in reverse transcription applications. Certain primer sets and targets can be used with Bst as a single-enzyme RT/DNA polymerase, but we recommend using a dedicated reverse transcriptase. If a single polymerase is desired, Bst 2.0 has significantly higher reverse transcriptase activity and should be used in place of Bst DNA polymerase LF.