FAQ: Does the Ph.D.™ Phage Display Cloning System come with double-stranded DNA vector?

Yes, M13KE is supplied in RF, or double-stranded, form in #E8101S that may be used directly in restriction digest. Single-stranded M13 DNA may be obtained by PEG/NaCl precipitation of phage particles from infected culture supernatants. Double-stranded DNA is purified from infected cells directly. A related product is the M13KE phage (#N0316S). Please see the Ph.D.™ Phage Display Manual for general M13 protocols.