HomeFAQsCan I use the wild type Mth RNA ligase included in the 5’ DNA adenylation kit instead of the Thermostable 5’AppDNA/RNA ligase for library construction?
FAQ: Can I use the wild type Mth RNA ligase included in the 5’ DNA adenylation kit instead of the Thermostable 5’AppDNA/RNA ligase for library construction?
No, ligation with the wild type Mth RNA ligase is inhibited by ATP. It is very efficient at adenylation but not ligation.
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