FAQ: Can I use the NEBNext Ultra DNA Library Prep Kit for Illumina reagents for preparing libraries for sequencing on the Roche 454 GS FLX & SOLiD 5500?

In theory yes. The workflow of the Ultra kit is compatible with any library prep that requires T/A single base overhang ligation. However, not all the reagents required for the Roche 454 & SOLiD 5500 library prep are included in this kit. In addition, we have only validated the Ultra kit on Illumina GAII, HiSeq and MiSeq platforms.