FAQ: T7 DNA Ligase is described as an enzyme that only ligates DNA fragments with cohesive ends. What length of cohesive end can T7 DNA Ligase ligate?

T7 DNA Ligase can ligate DNA fragments with cohesive ends that are 2 bps or longer. T7 DNA Ligase ligates 1 bp overhangs inefficiently. T7 DNA Ligase does not effectively ligate blunt end DNA fragments, except under reaction conditions of very high PEG concentrations (20-30% w/v).