HomeFAQsCan the Instant Sticky-end Ligase Master Mix be used for the ligation of blunt-end or single-base overhang fragments?
FAQ: Can the Instant Sticky-end Ligase Master Mix be used for the ligation of blunt-end or single-base overhang fragments?
Yes, but the reaction will no longer be instantaneous. An incubation time of 15-30 minutes at room temperature is required to ensure high efficiency ligation of blunt ends or single-base overhangs.
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