FAQ: Can the Blunt/TA Ligase Master Mix be used for the ligation of sticky-end fragments?

Yes. Blunt/TA Ligase Master Mix has been optimized for the ligation of blunt-end and single-base overhangs. Sticky-end fragment ligation, a reaction that proceeds with higher efficiency, can also be accomplished with this product.

Blunt/TA Ligase Master Mix improves yields for ends that typically react slowly

Yields of final ligation product for all reaction conditions using high concentration T4 DNA Ligase (NEB #M0202), the Quick Ligation Kit (NEB #M2200) and Blunt/TA Master Mix (NEB #M0367). Nick, cohesive end and 3´ single-base overhang substrates were incubated for 15 minutes; the 5´ single-base overhang was incubated for 1 hour.