HomeFAQsDuring size selection on 6% PAGE gel, which bands should I cut out of the gel?
FAQ: During size selection on 6% PAGE gel, which bands should I cut out of the gel?
The 140 and 150 bp bands correspond to adaptor-ligated constructs derived from the 21 and 30 nucleotide RNA fragments, respectively. The miRNA libraries run at 140 bp corresponding to 21 nucleotides and piRNA libraries run at 150 bp corresponding to 30 nucleotides.
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