FAQ: Does the PreCR Repair Mix remove covalent modifications from DNA bases, such as biotin or digoxigenin? Does it repair mismatches/ extra bases in DNA?

This repair mix will not remove covalent modification in the DNA bases. It will repair basic DNA damages such as abasic sites, nicks, thymine dimers, deaminated cytosine, oxidized guanine and pyrimidine. It also polishes 3' to hydroxyl if there is a block at 3' end. If the mismatches fall in those categories, yes, PreCR will repair it. Otherwise, no.