FAQ: How does the level of star activity of KpnI-HF compare to KpnI?

The Fidelity Index which measures the minimum units to produce star activity was determined using the following conditions; 1X NEBuffer, 5% glycerol, lambda DNA (1µg/50 µl), incubated for 1 hour at 37˚C . The minimum number of units of KpnI-HF required to produce star activity in the recommended rCutSmart Buffer™ is (≥250 units). The minimum number of units of KpnI required to produce star  activity in the recommended NEBuffer r1.1 is (16 units).The minimum number of units of KpnI required to produce star activity in rCutSmat Buffer is (4 units).

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Related Products:
KpnI-HF®