FAQ: What is the most convenient method of using BamHI with another enzyme that requires a low salt buffer?

Perform the first digest in the low salt buffer. Then supplement with 100–150mM NaCl using a concentrated NaCl stock solution (i.e. 5 M NaCI). Alternatively, perform the low salt digest in a 20 µl reaction volume and then increase the reaction volume to 50 µl, by adding 5 µl 10X NEBuffer r3.1 BamHI, and water.

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