HomeFAQsWhy does NEB offer K. lactis protease deletion strains?
FAQ: Why does NEB offer K. lactis protease deletion strains?
In all yeasts, host secetory pathway proteases may adversely affect the quality of a secreted recombinant protein. In K. latics, the offending protease is most often an apartyl protease from the protein family pfam00026. We developed an isogenic set of four aspartyl protease deletion strains as a means to address detrimental proteolysis. Each strain harbors a marker-free deletion of a single K. latics gene encoding a secretory pathway aspartyl protease that was identified by bioinformatic analysis of genomic sequence.
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