Home FAQs Does SssI Methyltransferase require magnesium in the buffer?

FAQ: Does SssI Methyltransferase require magnesium in the buffer?

No. With magnesium the enzyme reaction is distributive and the enzyme exhibits topoisomerase activity. Without magnesium the reaction is processive (2).

(2) Matsuo et al, Nucl. Acids Res. (1994) Vol 22, 5354-5359.