FAQ: Is there any difference in the methylation sensitivity between PvuII-HF and PvuII?

Both PvuII and PvuII-HF are are blocked by complete EcoBI methylation. PvuII-HF is blocked by EcoBI hemimethylation while PvuII is impaired. For more specific information regarding methylation, follow the link to the Restriction Enzyme database REBASE.

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