FAQ: How does Tth Endonuclease IV perform in PCR?

Tth EndoIV can survive typical PCR cycling. The percentage surviving will depend on the specific conditions. It has a significantly higher thermostability in ThermoPol buffer than Standard Taq buffer and we recommend adding Zn++ (usually ZnCl2) to the reaction to 25 micromolar to ensure the enzymes thermostability. We have not seen this level of Zn++ to effect PCR reactions.