HomeFAQsCan T4 DNA ligase be used to ligate across an abasic site?
FAQ: Can T4 DNA ligase be used to ligate across an abasic site?
T4 DNA Ligase will not ligate across and AP site. The PreCR Repair Mix will fix the AP site (in dsDNA only), add the proper base, and then ligate the dsDNA structure together.
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