HomeFAQsCan phi29 DNA Polymerase be used in other NEBuffers?
FAQ: Can phi29 DNA Polymerase be used in other NEBuffers?
Yes. Phi29 DNA Polymerase is 30% active in NEBuffer 1, 35% active in NEBuffer 2, 25% active in NEBuffer 3, 90% active in NEBuffer 4, and 30% active in ThermoPol Buffer. It also is 100% active in rCutSmart buffer with the addition of 4mM DTT (final concentration).
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