HomeFAQsWhy are there larger than expected bands after digestion with the KpnI-HF restriction enzyme?
FAQ: Why are there larger than expected bands after digestion with the KpnI-HF restriction enzyme?
The increased specificity for the KpnI-HF cut site has increased binding of the enzyme to the DNA and the enzyme may remain attached to the DNA during gel electrophoresis. To disrupt binding, add SDS to a final concentration of 0.5% - 1% or purify DNA before electrophoresis.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.