FokI is used to cleave between any two base pairs. See Podhajska, A.J., Szybalski, W. (1985). Gene 40: 175-182 In brief, you synthesize an oligonucleotide which contains a double-stranded FokI site and a single-stranded extension which anneals to the DNA template at the point where you would like to cut. Anneal the oligonucleotide duplex to the single stranded DNA template. In the subsequent cleavage reaction, the FokI will bind to the double-stranded site on teh oligonucleotide duplex, and will cut the template to which it is annealed.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
