FAQ: What Poly(U) length can be expected to be incorporated by Poly(U) Polymerase?

In the standard recommended reaction the tailing length for the Poly(U) polymerase is a function of both accessible primer and UTP concentration. Using a primer where 100% of the population is extended, a typical 30 minute incubation with 0.5 mM UTP will extend all RNA molecules approximately 1,000 bases.