FAQ: When using PURExpress, I was able to synthesize the target protein, but full-length product is not major species?

Translation initiation and/or termination not correct
The production of full-length protein requires proper initiation and termination. Internal ribosome entry sites and/or premature termination can produce unwanted truncated proteins. Initiation at non-authentic AUG codons and premature termination are difficult to control. If many rare codons are present or the target has an unusually high percentage of a particular amino acid, supplementation of the “missing” tRNA may help.