FAQ: Why is induced protein insoluble (C2566)?
» Induce at lower temperatures (as low as 12-15°C overnight)
» Reduce IPTG concentration to 0.01 - 0.1 mM
» Induce for less time (as little as 15 minutes)
» Induce earlier in growth (OD600 = 0.3 or 0.4)