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FAQ: Why is induced protein insoluble (C2528)?

Check for insolubility – this is important because uncontrolled T7 expression often leads to very high production of protein that can result in the target protein becoming insoluble. Solutions around this are:
  • Induce at a lower temperature (20 to 30°C)
  • Induce earlier in growth phase (OD600 = 0.3 or 0.4)
  • Test expression using a full range of L-rhamnose concentrations: 0 to 2,000 µM (see figure on the main product page).