HomeFAQsWhy are there no colonies or no growth in liquid culture (C3022)?
FAQ: Why are there no colonies or no growth in liquid culture (C3022)?
Even though T7 expression is tightly regulated, there may be a low level of basal expression in the T7 Express Crystal host. If toxicity of the expressed protein is likely, incubate at 30°C or room temperature to alleviate toxicity issues. In addition, check antibiotic concentration (test with control plasmid).