HomeFAQsWhat is the optimal heat shock time for this strain (NEB #C2992H and NEB #C2992I)?
FAQ: What is the optimal heat shock time for this strain (NEB #C2992H and NEB #C2992I)?
Heat shock at 42ºC for 30 seconds results in the highest transformation efficiency for NEB 5-alpha F´Iq competent E.coli (NEB #C2992H and NEB #C2992I). Expect approximately 70% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.