FAQ: What is the optimal heat shock time for this strain (NEB #C2523H and NEB #C2523I)?

Heat shock at 42ºC for 20 seconds results in the highest transformation efficiency for NEBExpress competent E.coli (NEB #C2523H and NEB #C2523I). Expect approximately 50% loss in transformation efficiency when heat shocking for 80 seconds (see Figure on the main product page).
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    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.