HomeFAQsI want to clone primer extension products made with Vent DNA Polymerase or Deep Vent DNA Polymerase. Are some protocols better than others?
FAQ: I want to clone primer extension products made with Vent DNA Polymerase or Deep Vent DNA Polymerase. Are some protocols better than others?
The best protocols depend on blunt-end cloning into a phosphatase-treated vector. The insert should be kinased at its 5' ends unless the primer has been synthesized with a 5' phosphate.
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