FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2988J)?

Incubating DNA with NEB 5-alpha competent cells (Subcloning Efficiency) on ice for 30 minutes is recommended. Expect approximately 30% loss in transformation efficiency when incubating for 10 minutes. (see Figure on the main product page).
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    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.