Home FAQs How long should I incubate cells on ice after DNA has been added (NEB #C3037H and NEB #C3037I)?

FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C3037H and NEB #C3037I)?

Incubating DNA with NEB Express Iq competent cells on ice for 30 minutes is recommended. Expect approximately 40% loss in transformation efficiency when incubating for 10 minutes (see Figure on the main product page).

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