FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2566H and NEB #C2566I)?

Incubating DNA with T7 Express competent cells on ice for 30 minutes is recommended. Expect approximately 30% loss in transformation efficiency when incubating for 10 minutes (see Figure on the main product page).
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    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.