Home FAQs How long should I incubate cells on ice after DNA has been added (NEB #C2523H and NEB #C2523I)?

FAQ: How long should I incubate cells on ice after DNA has been added (NEB #C2523H and NEB #C2523I)?

Incubating DNA with NEB Express competent cells on ice for 30 minutes is recommended. When tested with pUC19, an incubation time of 20 minutes did not hurt the transformation efficiency (see Figure on the main product page).

Videos

  1. TransformationTips_VideoThumb_282x210_0616

    Tips for Successful Transformations with NEB® Competent Cells

    Follow these tips to get superior results.