FAQ: Can I use α1-3,6 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?

Yes. We recommend using 1X GlycoBuffer 2 for digestion of α1-3,6 Galactosidase with PNGase F and/or O-Glycosidase. First digest your glycoprotein with PNGase F or O-Glycosidase, heat-kill the enzyme at 65°C for 10 min, and then treat with α1-3,6 Galactosidase in the same 1X GlycoBuffer 2.