HomeFAQsAre the DNA fragments produced by Deep Vent (exo-) DNA Polymerase blunt-ended or do they have the single-base 3' overhang that Taq DNA Polymerase yields?
FAQ: Are the DNA fragments produced by Deep Vent (exo-) DNA Polymerase blunt-ended or do they have the single-base 3' overhang that Taq DNA Polymerase yields?
There are predominantly two kinds of ends seen; 70% are blunt-ended, while 30% are predominantly single base 3' overhangs. If >95% blunt-ended fragments are desired, use a proofreading DNA Polymerase such as Deep Vent DNA Polymerase (NEB# M0258 ).
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