FAQ: Can we use the ribonucleotide Mix (N0466) to replace the addition of an rATP solution for the poly A tailing of an RNA template by E.coli Poly(A) polymerase?

The use of the rNTP mix in an E.coli Poly(A) polymerase reaction is not recommended because nucleotides other than ATP will not be incorporated by the Poly(A) polymerase and they potentially can inhibit the reaction. We sell rATP as a separate product (P0756S).