Rapid Generation of sgRNA
Return to Genome EditingThe EnGen sgRNA Synthesis Kit simplifies the generation of microgram quantities of custom sgRNAs in an hour or less by combining template synthesis and transcription. The single-tube reaction is easy to set up and requires a single ~55 nt ssDNA target-specific oligonucleotide, which is combined with the Reaction Mix and Enzyme Mix included in the kit. Target-specific DNA oligos are configured using NEB’s EnGen sgRNA Template Oligo Designer (accessible through NEBioCalculator®) and can be ordered from any DNA oligo supplier. sgRNAs are suitable for use in downstream applications, including CRISPR/Cas9-based genome editing and in vitro DNA cleavage. This single-reaction format offers ease-of-use and eliminates separate DNA amplification and template clean up steps.
EnGen sgRNA Synthesis Kit Overview

T7 transcription
