Whole Genome Amplification (WGA) is a method for robust amplification of an entire genome, starting with nanogram quantities of DNA and resulting in microgram quantities of amplified products. WGA has become an invaluable method for preserving limited samples of precious stock material, particularly when using WGA methods that have been developed to amplify material from a single cell. Several methods have been developed for high-fidelity whole genome amplification, including Multiple Displacement Amplification (MDA), Degenerate Oligonucleotide PCR (DOP-PCR) and Primer Extension Preamplification (PEP). While DOP-PCR and PEP are based on standard PCR techniques, MDA can be achieved with an isothermal reaction setup.
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