DNA Amplification

Multiplex PCR

Multiplex PCR involves the simultaneous amplification of two or more primer sets in a single reaction. Because of the increased number of primers in the reaction, proper primer design is critical to the success of your multiplex reaction. Specialized enzyme formulations can also increase multiplex performance and reduce the need for troubleshooting. NEB offers both Taq-based and Q5®-based formulations that are appropriate for use in multiplex PCR.

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Protocols for Multiplex PCR
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Multiplex PCR
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Deoxynucleotide (dNTP) Solution Mix

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Deoxynucleotide (dNTP) Solution Set

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Hot Start Taq 2X Master Mix

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Hot Start Taq DNA Polymerase

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LongAmp® Hot Start Taq 2X Master Mix

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Multiplex PCR 5X Master Mix

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OneTaq® 2X Master Mix with Standard Buffer

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OneTaq® DNA Polymerase

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OneTaq® Hot Start 2X Master Mix with Standard Buffer

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OneTaq® Hot Start DNA Polymerase

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OneTaq® Hot Start Quick-Load® 2X Master Mix with GC Buffer

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OneTaq® Quick-Load® DNA Polymerase

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OneTaq® RT-PCR Kit

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OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer

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OneTaq® Quick-Load® 2X Master Mix with Standard Buffer

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Phusion GC Buffer Pack

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Phusion High-Fidelity DNA Polymerase

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Phusion High-Fidelity PCR Master Mix with GC Buffer

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Phusion High-Fidelity PCR Master Mix with HF Buffer

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Phusion Hot Start Flex 2X Master Mix

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Phusion Hot Start Flex DNA Polymerase

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Phusion High-Fidelity PCR Kit

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Q5® High-Fidelity 2X Master Mix

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Q5® High-Fidelity DNA Polymerase

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Q5® High-Fidelity PCR Kit

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Q5® Hot Start High-Fidelity 2X Master Mix

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Q5® Hot Start High-Fidelity DNA Polymerase

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Q5® Reaction Buffer Pack

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Q5®-XT Hot Start High-Fidelity 2X Master Mix

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Q5U® Hot Start High-Fidelity DNA Polymerase

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Quick-Load® Taq 2X Master Mix

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Taq 2X Master Mix

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Taq 5X Master Mix

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Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

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Taq DNA Polymerase with Standard Taq Buffer

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Taq DNA Polymerase with ThermoPol® Buffer

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Taq PCR Kit